ABSTRACT
OBJECTIVE：To sepa rate and identify the phenols compounds in Aurantii Fructus Immaturus ，and to provide reference for basic research of its effective substances. METHODS ：The phenols compounds were isolated and separated by silica gel，HW-40F gel column ，ODS reversed column and preparation HPLC. Their physicochemical properties and spectral data were used to identify the structures. RESULTS & CONCLUSIONS ：Totally 12 compounds were isolated from Aurantii Fructus Immaturus，identified as 6′-O-trans- cinnamoyl- 3，5-dihydroxyphenyl β-D-glucopyranoside（compound 1），methyl 3-（2′，4′- dihydroxy phenyl ）propionate（compound 2），phloroglucinol（compound 3），aurantiside A （compound 4），5，7，4′-trihydroxy-8， 3′-dimethoxyflavone-3-O-6″-（3-hydroxyl-3-methylglutaroyl） β-D-glucopyranoside （compound 5）， aromadendrin-7-O-β-D- glucopyranside（compound 6），hesperidin-7-O-β-D-glucoside（compound 7），naringin（compound 8），hesperidin（compound 9）， narirutin （compound 10），neoeriocitrin （compound 11），eriocitrin （compound 12）. Among them ，compound 1 is a new compound，and compounds 2 and 3 are isolated from Critus for the first time.
ABSTRACT
OBJECTIVE: To study the chemical constituents of ethanol extract from Cirtus aurantium. METHODS: The ethanol extract of C. aurantium was isolated and purified by silica gel column, open ODS column, preparation liquid chromatography, HW-40F gel column and macroporous resin column. The structure of compounds was analyzed and identified according to physicochemical properties and spectral data (mass spectrum, hydrogen spectrum, carbon spectrum). RESULTS: Eight compounds were isolated from ethanol extract of C. aurantium, i. e. Rimboxo (1), Thymidine (2), Uracil (3), Cyclo-(Leu1-Ile2-Ala3-Thr4-Gly5-Thr6-Phe7) (4), Cyclo- (Leu1-Leu2-Pro3-Tyr4-Gly5-Ser6-Pro7) (5), Meranzin hydrate-β-D-glucoside (6), Umbelliferone (7), Linaloyl glucoside (8). CONCLUSIONS: Compound 1, 2, 3, 8 are isolated from Citrus L. for the first time, compound 4, 5, 6 were isolated from C. aurantium for the first time. The study lays the foundation for quality evaluation of C. aurantium.